Evaluating the effect of chromosomal context on zinc finger nuclease efficiency

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Evaluating the effect of chromosomal context on zinc finger nuclease efficiency

Introduction Zinc Finger Nuclease (ZFN) technology has provided researchers with a tool for integrating exogenous sequences into most cell lines or genomes in a precise manner. Using current methods, the efficiency of targeted integration (TI) into the host genome is generally low and is highly dependent on the ZFN activity at the genomic locus of interest. It is unknown if the ZFN binding and ...

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ژورنال

عنوان ژورنال: BMC Proceedings

سال: 2013

ISSN: 1753-6561

DOI: 10.1186/1753-6561-7-s6-p3